This should be taken into account when designing studies and when considering the findings of existing studies.Ĭhemoprevention represents an attractive modality against colorectal cancer (CRC) although widespread clinical implementation of promising agents (e.g. Suppression or elevation of proliferation parameters by bowel preparation may mask any effect due to an intervention or disease.Ĭommonly used bowel preparations may have significant and different effects on crypt cell proliferation. A similar trend was seen for whole-mount mitotic figure counts. There were significant differences in labelling indices (LI) between biopsies taken after Klean-prep and those taken after Picolax preparation, for both Ki67 (p = 0.019) and pHH3 (p = 0.017). Parameters were compared between groups using analysis of variance and paired t-tests. Subsequent biopsies were obtained from the same patients without bowel preparation and similarly assessed. Crypt cell proliferation was assessed by whole-mount mitotic figure count, and by two different immunohistochemical (IHC) labelling methods (Ki-67 and pHH3). Modulation of cell proliferation by bowel preparation with oral laxatives may confound the findings of such studies, but there is little data on the impact of commonly used bowel preparations on markers of cell proliferation.Ĭrypt length, crypt cellularity and crypt cell proliferation were assessed in biopsies acquired after preparation with either Klean-Prep or Picolax. Human studies often rely on the assessment of tissue acquired at endoscopy. Markers of crypt cell proliferation are frequently employed in studies of the impact of genetic and exogenous factors on human colonic physiology.
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